|
| Status |
Public on Aug 19, 2009 |
| Title |
Gallus gallus bursal IFE_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
bursal FAE
|
| Organism |
Gallus gallus |
| Characteristics |
source type: isolated cell
organism part: bursa of Fablicius
cell type: interfollicular epithelium
strain[background]: Line-M
developmental stage: chick
age: 19 days
microbial condition: SPF
disease state: healthy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted with commercial kit.
|
| Label |
biotin
|
| Label protocol |
Two-Cycle Target Labeling and Control Reagents
|
| |
|
| Hybridization protocol |
Affymetrix standard procedures
|
| Scan protocol |
using GeneChip Scanner 3000
|
| Description |
Bursa of Fablicius was dissected from the rectum and soaked in Hank's Buffered Salt Solution (HBSS) (GIBCO) containing black ink for 5 min at room temperature to visualize FAE. After incubation, the tissue was treated with 40 mM EDTA/25 mM Hepes (pH 7.4) in HBSS for 5min at room temperature to collect bursal FAE or 30 min at 37ºC for bursal IFE. Bursal FAE and IFE were collected with a fine 29G needle under stereomicroscope monitoring.
|
| Data processing |
Raw signals are from MAS5. Normalized data from GeneSpring 7.3.1.
|
| |
|
| Submission date |
May 13, 2009 |
| Last update date |
Aug 19, 2009 |
| Contact name |
Shinji Fukuda |
| E-mail(s) |
[email protected]
|
| Organization name |
Keio University
|
| Department |
Institute for Advanced Biosciences
|
| Lab |
Microbiome Research Group
|
| Street address |
246-2 Mizukami, Kakuganji
|
| City |
Tsuruoka |
| State/province |
Yamagata |
| ZIP/Postal code |
997-0052 |
| Country |
Japan |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE16081 |
Gallus gallus bursal FAE and IFE |
|
