|
| Status |
Public on Jun 30, 2020 |
| Title |
RNA-seq of LNCaP/MYC KD 637 Rep 2 |
| Sample type |
SRA |
| |
|
| Source name |
prostate cancer
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LNCaP
condition: MYC Knockdown Construct 637
replicate: 2
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted from cells using the Qiagen RNeasy Mini Plus kit
1 microgram of RNA was extracted from cells and assembled into PolyA-selected libraries using the Illumina TruSeq Stranded Total RNA Kit.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
A2-105-16
|
| Data processing |
Libraries were sequenced with 125 cycles paired-end. FASTQ files were aligned with RUM to hg19 and sorted with PICARD.
FASTQ files were aligned with RUM to hg19 and sorted with PICARD.
Genome_build: hg19
Supplementary_files_format_and_content: tab separated values for gene-level FPKM
|
| |
|
| Submission date |
Aug 18, 2019 |
| Last update date |
Jul 01, 2020 |
| Contact name |
Adam Sowalsky |
| Organization name |
National Cancer Institute
|
| Department |
LGCP
|
| Lab |
Sowalsky Lab
|
| Street address |
37 Convent Drive Building 37 Room 1062B
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE135942 |
Genetic studies of MYC in prostate cancer cell lines |
|
| Relations |
| SRA |
SRX6717244 |
| BioSample |
SAMN12574710 |
