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Sample GSM4061930 Query DataSets for GSM4061930
Status Public on Feb 08, 2020
Title Sample 127458: WTICD1
Sample type SRA
 
Source name lung
Organism Cricetulus griseus
Characteristics genotype: Beta1-ICD
tissue: lung
Treatment protocol No treatment
Growth protocol Incubator; 37 degrees C and 5% CO2
Extracted molecule polyA RNA
Extraction protocol Qiagen RNeasy Plus Mini Kit
stranded TruSeq mRNA (poly A selection).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing RNA-Seq was performed using Illumina NovaSeq-6000, paired-end 50 cycle
Base-calling software: Illumina Casava 1.8 software
Alignment was conducted using TopHat (version 2.0.13) and Bowtie (version 2.2.1), with default parameter settings, with exception of: "---b2-very-sensitive" for additional search of valid alignments
Expression quantitation was performed with HTSeq
Genome_build: CriGri_1.0
Supplementary_files_format_and_content: htseq raw counts
 
Submission date Sep 05, 2019
Last update date Apr 06, 2020
Contact name Bioinformatics Core
E-mail(s) [email protected]
Organization name University of Michigan
Street address BRCF Bioinformatics Core, 2800 Plymouth Rd
City Ann Arbor
State/province Mi
ZIP/Postal code 48109-2800
Country USA
 
Platform ID GPL27425
Series (1)
GSE136927 Voltage-gated sodium channel (VGSC) beta1 subunit-intracellular domain (Beta1-ICD) overexpression in Chinese hamster lung cells
Relations
BioSample SAMN12699083
SRA SRX6805922

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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