|
| Status |
Public on Nov 01, 2019 |
| Title |
iWAT sample.hfd_con2 |
| Sample type |
SRA |
| |
|
| Source name |
inguinal white adipose tissue
|
| Organism |
Mus musculus |
| Characteristics |
diet/treatment: High Fat Diet
genotype: control (MRflox/flox )
tissue: inguinal white adipose tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissues were flash frozen in liquid nitrogen. RNA was extracted from tissues using TRIzol Reagent (Ambion) and Purelink™ RNA Mini Kit (Invitrogen™). RNA integrity was validated by an Agilent bioanalyzer.
KAPA RiboErase
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Basecalling and demultiplexing with Illumina RTA 3.4
Fastq files were aligned with STAR 2.5.1a
Gene counts dervied with Subread:featureCount 1.4.6p-5 and annotated with Ensembl Biomart
Genome_build: Ensembl GRCm38.76
Supplementary_files_format_and_content: Gene counts, CPMs, RPKMs in tab-delimited text format
|
| |
|
| Submission date |
Oct 09, 2019 |
| Last update date |
Nov 02, 2019 |
| Contact name |
Charles A Harris |
| E-mail(s) |
[email protected]
|
| Phone |
3143623802
|
| Organization name |
Washington University School of Medicine in St. Louis
|
| Department |
Department of Medicine
|
| Lab |
Harris Lab
|
| Street address |
660 S. Euclid Ave
|
| City |
St Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE138632 |
Role of Mineralocorticoid Receptor (Nr3c2) in Adipogenesis and Obesity in Male Mice |
|
| Relations |
| BioSample |
SAMN12999997 |
| SRA |
SRX6969277 |
