|
| Status |
Public on Jan 28, 2021 |
| Title |
V043_F047 |
| Sample type |
SRA |
| |
|
| Source name |
Human blood cells from a nonsmoking adult
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Blood
smoker: no
|
| Treatment protocol |
Cryopreserved buffy coat was CD15 depleted and cells were resuspended in PBS with BSA.
|
| Growth protocol |
Human whole blood was collected from smoking and nonsmoking adults
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Chromium™ Single Cell 3' Library & Gel Bead Kit v2 (10X Genomics) according to the manufacturer’s guidelines
Chromium™ Single Cell 3' Library & Gel Bead Kit v2 (10X Genomics) according to the manufacturer’s guidelines
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Filtered gene read count matrix obtained using Cell Ranger version 2.0.2
Genome_build: hg19
Supplementary_files_format_and_content: counts: Cell Ranger filtered gene read count matrix in Matrix Market format
|
| |
|
| Submission date |
Oct 15, 2019 |
| Last update date |
Jan 28, 2021 |
| Contact name |
Douglas A. Bell |
| E-mail(s) |
[email protected]
|
| Organization name |
National Institute of Environmental Health Sciences
|
| Department |
Immunity Inflammation and Disease Lab
|
| Street address |
PO Box 12233
|
| City |
Research Triangle Park |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE138867 |
Single-cell RNA sequencing of peripheral blood immune cells from smokers and nonsmokers |
|
| Relations |
| BioSample |
SAMN13031840 |
| SRA |
SRX6992758 |
