|
| Status |
Public on Nov 01, 2022 |
| Title |
2_WT |
| Sample type |
SRA |
| |
|
| Source name |
Kidney
|
| Organism |
Mus musculus |
| Characteristics |
genotype: WT
kidney pair: A
|
| Treatment protocol |
Mice were infected with UPEC trans urethrealy twice 1 hour apart and culled 6 hours later.
|
| Growth protocol |
Mice were housed under SPF conditions]
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples were stored in RNAlater until processing. Samples were homoginised in lysis buffer (Ambion) and RNA extracted with Purelink RNA mini kit (Ambion)
Libraries were constructed with Illumina TruSeq total Stranded RNA library prep kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Demultiplex with Casava
Alignment with Hisat2
Genome_build: Mm10
|
| |
|
| Submission date |
Nov 01, 2019 |
| Last update date |
Nov 01, 2022 |
| Contact name |
John Robert Ferdinand |
| Organization name |
University of Cambridge
|
| Street address |
Department of Medicine
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0QH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE139848 |
Analysis of UTI on WT and muMT KO mice |
|
| Relations |
| BioSample |
SAMN13181472 |
| SRA |
SRX7087490 |
