|
Status |
Public on Mar 09, 2020 |
Title |
nip1_tb1601_rep1_totalRNA |
Sample type |
SRA |
|
|
Source name |
YA (young adults)
|
Organism |
Caenorhabditis elegans |
Characteristics |
age: YA (young adults) genotype: nip1_tb1601
|
Growth protocol |
C. elegans was grown at 20C, with HB101 bacteria on agar plates.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol-Dnase NEB NEXT Ultra
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 1500 |
|
|
Description |
nipmutants_totalRNApolyA_TE.txt
|
Data processing |
Trimmed reads were aligned to ce11 reference genome using the STAR aligner with multipmapping options. Read counts on exons was extracted using the FeatureCounts with --fraction option. data was normalized with DeSEQ2. heatmaps and volcano plots were generated with a custom script on Rstudio. Genome_build: ce11 Supplementary_files_format_and_content: bam, txt
|
|
|
Submission date |
Nov 29, 2019 |
Last update date |
Mar 09, 2020 |
Contact name |
Eric Miska |
E-mail(s) |
[email protected]
|
Organization name |
University of Cambridge
|
Department |
The Gurdon Institute
|
Lab |
Eric Miska Lab
|
Street address |
tennis court road
|
City |
cambridge |
ZIP/Postal code |
CB2 1QN |
Country |
United Kingdom |
|
|
Platform ID |
GPL18730 |
Series (1) |
GSE126377 |
RNA memories: trans-generational epigenetic inheritance of RNA-mediated gene silencing |
|
Relations |
BioSample |
SAMN13428638 |
SRA |
SRX7241750 |