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Sample GSM4196583 Query DataSets for GSM4196583
Status Public on Mar 09, 2020
Title nip1_tb1601_rep1_totalRNA
Sample type SRA
 
Source name YA (young adults)
Organism Caenorhabditis elegans
Characteristics age: YA (young adults)
genotype: nip1_tb1601
Growth protocol C. elegans was grown at 20C, with HB101 bacteria on agar plates.
Extracted molecule total RNA
Extraction protocol Trizol-Dnase
NEB NEXT Ultra
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
 
Description nipmutants_totalRNApolyA_TE.txt
Data processing Trimmed reads were aligned to ce11 reference genome using the STAR aligner with multipmapping options.
Read counts on exons was extracted using the FeatureCounts with --fraction option.
data was normalized with DeSEQ2.
heatmaps and volcano plots were generated with a custom script on Rstudio.
Genome_build: ce11
Supplementary_files_format_and_content: bam, txt
 
Submission date Nov 29, 2019
Last update date Mar 09, 2020
Contact name Eric Miska
E-mail(s) [email protected]
Organization name University of Cambridge
Department The Gurdon Institute
Lab Eric Miska Lab
Street address tennis court road
City cambridge
ZIP/Postal code CB2 1QN
Country United Kingdom
 
Platform ID GPL18730
Series (1)
GSE126377 RNA memories: trans-generational epigenetic inheritance of RNA-mediated gene silencing
Relations
BioSample SAMN13428638
SRA SRX7241750

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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