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| Status |
Public on Jan 15, 2020 |
| Title |
HF_A |
| Sample type |
SRA |
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| Source name |
anagen skin
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| Organism |
Capra hircus |
| Characteristics |
tissue: skin
strain: Albas White Cashmere Goat
developental stage: anagen
Sex: female
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| Extracted molecule |
total RNA |
| Extraction protocol |
For each group skin tissues were obtained from at least 3 independent female, prior to digestion. To prepare dorsal skin single cell suspension for single-cell RNA sequencing, the back skin tissues were isolated via microdissection and 0.25 % trypsin/EDTA solution was used to digest dorsal skin tissues at 37 °C for 30 min，mechanically dissociate the tissue once every 10 min. 1 mg/ml collagenase IV (Sigma, St Louis, MO, USA) was used to digest the tissue which digested by trypsin at 37 °C for 30 min. the skin tissues were mechanically dissociated into single cell suspension by pipetting, the cell suspensions were then filtered through a 30 μm nylon cell strainer, to remove villus debris(BD Falcon, BD Biosciences, San Jose, CA, USA) prior to single cell library construction.
Single-cell RNA-seq libraries were prepared using Single Cell 3’ Library Gel Bead Kit V2 (10x Genomics Inc., Pleasanton, CA, USA, Cat#120237) following the manufacture’s introduction.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
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| Description |
The group information is prefixed with HF-A in column of processed data file
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| Data processing |
We used CellRanger count pipeline 2.2.0 to analyze the sequencing data and generated the single cell information. To generate same number of total cells for downstream analysis, we used augments ‘--force-cells=7000 \’.
Genome_build: ARS1
Supplementary_files_format_and_content: tab-delimited text files stores normalized and log-transformed single cell expression value
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| Submission date |
Dec 02, 2019 |
| Last update date |
Jan 15, 2020 |
| Contact name |
Feng Yang |
| E-mail(s) |
[email protected]
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| Organization name |
Inner Mongolia Agricultural University
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| Street address |
306 Zhaowuda Road
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| City |
Hohhot |
| State/province |
Inner Mongolia |
| ZIP/Postal code |
010000 |
| Country |
China |
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| Platform ID |
GPL15473 |
| Series (1) |
| GSE141284 |
The function of DP cells during the development of hair follicles in cashmere goats |
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| Relations |
| BioSample |
SAMN13446885 |
| SRA |
SRX7255709 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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