|
| Status |
Public on Dec 31, 2020 |
| Title |
Patient 05 A05 |
| Sample type |
RNA |
| |
|
| Source name |
Recurrent GBM
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: Recurrent glioblastoma
tissue: Glioblastoma tumor
Sex: Female
age: 48
os: Dead
os time (month): 17
pfs: Progression
fps time (month): 5
outcome: Poor response
|
| Treatment protocol |
Not available.
|
| Growth protocol |
Not available.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from tumor tissues of patients with recurrent GBM prior to AKC treatment.
|
| Label |
NanoString Oligonucleotide Probe
|
| Label protocol |
The RNA samples were run on the NanoString nCounter Analysis System (NanoStringTechnologies, Inc., WA, USA) according to the manufacturer's directions.
|
| |
|
| Hybridization protocol |
5μL (100-300ng) of each RNA sample was mixed with 8μL of the Master Mix (reporter codeSet and hybridization buffer). 2μL of the capture probeSet was added; the solution was mixed and spun down. It was placed in a 65°C thermocycler (Bio-Rad Laboratories Inc, Hercules, California, USA) for 16 hours (maximum hybridization time should not exceed 48 hours).
|
| Scan protocol |
The samples were transferred to the preparation station (NanoString Technologies, Seattle, WA) with prepared nCounter Master Kit and a cartridge. The samples binding to the cartridge. The preparation station 12 lanes per run in approximately 2.5 to 3 hours. The cartridges were transferred to the Digital Analyzer (NanoString Technologies, Seattle, WA) for analysis. Cartridges were then scanned on the Digital Analyzer at 555 fields of view.
|
| Description |
A05
750 mRNA
Log2 normalized counts
|
| Data processing |
To quantify gene expression values, first normalization step was analyzed by geNormalgorithm in nCounter Advanced Analysis ver2.0.115.
|
| |
|
| Submission date |
Dec 28, 2019 |
| Last update date |
Jan 01, 2021 |
| Contact name |
Kyunggi Cho |
| E-mail(s) |
[email protected]
|
| Phone |
821052843097
|
| Organization name |
CHA bundang medical center
|
| Department |
neurosurgery
|
| Street address |
yatap 59
|
| City |
seongnam |
| ZIP/Postal code |
13496 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL27956 |
| Series (1) |
| GSE142693 |
Identification of mode of action for cell-based immunotherapy with ex-vivo expanded and activated autologous killer cells (AKCs) to recurrent glioblastoma (GBM) patients |
|
