|
| Status |
Public on Dec 30, 2010 |
| Title |
Patient ID_208 |
| Sample type |
RNA |
| |
|
| Source name |
Liver, ID_208
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: liver (chronic hepatitis)
drug-response: non-virological responder
age (years): 70
sex: Male
type of hcv: 1B
degree of inflammation/fibrosis: A2/F2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by using mirVana™ miRNA Isolation Kit (Ambion) according to manufacturer’s instruction
|
| Label |
Cy3
|
| Label protocol |
100ng total RNA was dephosphorylated at the presence of calf intestine alkaline phsphatase, then a Cy3-pCp molecule was then ligated to the 3' end of the RNA molecule according to Agilent miRNA protocol version 1.0
|
| |
|
| Hybridization protocol |
labeled RNA was hybridized to Agilent miRNA array ( 8 arrays per slide) at 55°C for 20h in rotisserie in a hybridization oven. Disassembled arrays was washed in GE wash buffer 1&2 for 5 minutes each.
|
| Scan protocol |
Images was scanned with Agilent scanner at 5µm resolution
|
| Data processing |
The raw data for same gene was then summarized automatically in Agilent feature extraction protocol to generate Gene view file, providing expression data for each gene probed on the array. Features interogating same miRNA were summarized together based on their weight. No interarray normalization method was employed.
|
| |
|
| Submission date |
Jul 01, 2009 |
| Last update date |
May 01, 2014 |
| Contact name |
Masami Tanaka |
| E-mail(s) |
[email protected]
|
| Organization name |
Tokyo Medical University
|
| Street address |
Sinjuku 6-1-1
|
| City |
Sinjuku-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
160-8402 |
| Country |
Japan |
| |
|
| Platform ID |
GPL9081 |
| Series (1) |
| GSE16922 |
MicroRNA expression profiling predicts clinical outcome of chronic hepatitis C by interferon administration |
|
