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| Status |
Public on Jan 01, 2021 |
| Title |
MCF-7 cells, siTRIM3_3 |
| Sample type |
SRA |
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| Source name |
MCF-7 cells
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| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF-7
genotype: TRIM3 depletion
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| Treatment protocol |
si RNA 50 nM
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| Growth protocol |
DMEM medium with 10% FBS
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction with Qiagen kit
NA
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
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| Data processing |
Removed reads with low quality, adapter contamination and high content of unknown base N
Aligned to the hg38 whole genome using HISAT2
Quanitify gene-expression level using RSEM,which is estimate by count and RPKM value
Genome_build: hg38
Supplementary_files_format_and_content: ExpressionProfile.txt containing RPKM values
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| Submission date |
Jan 14, 2020 |
| Last update date |
Jan 01, 2021 |
| Contact name |
Le Wu |
| E-mail(s) |
[email protected]
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| Organization name |
HuaZhong Agricultural university
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| Street address |
No.1,Shizishan Street · Hongshan District · Wuhan
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| City |
Wu han |
| State/province |
Hu bei |
| ZIP/Postal code |
430000 |
| Country |
China |
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| Platform ID |
GPL23227 |
| Series (1) |
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| Relations |
| BioSample |
SAMN13841708 |
| SRA |
SRX7552717 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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