Frozen tissues were homogenized in Trizol (Invitrogen Life Technologies, Gaithersburg, MD, USA). After extraction with isopropanol, total RNAs were purified on RNeasy Qiagen columns (Qiagen Inc., Valencia, CA, USA).
Label
Biotin-Affy
Label protocol
Affy-FS450-0007
Hybridization protocol
Affy-169 : 300.0 ng at 45 degree_C during 17 hours
Scan protocol
Arrays were scanned using the GeneChip Scanner 3000 7G (Affymetrix).
Description
2827 TG Date:2008/02/08
Data processing
Interslide norm. : none, Intraslide norm. : none, Background norm. : rma, Software used : expr_console --> VALUE data obtained from log2 normalized expression signal.
Submission date
Jul 13, 2009
Last update date
Jul 15, 2009
Contact name
Kevin Lebrigand
Organization name
IPMC/CNRS
Lab
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
