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Sample GSM4306909 Query DataSets for GSM4306909
Status Public on Feb 12, 2020
Title 20_02_2013_H12_S29.CEL healthy control
Sample type RNA
 
Source name whole blood
Organism Homo sapiens
Characteristics tissue: blood
disease state: healthy control
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using a PAXGene Blood RNA kit (PreAnalytiX GmbH) according to the manufacturer’s instructions.
Label biotin
Label protocol Gene expression analysis was performed using the Thermo Fischer Scientific Human Genome U219 96-array plate. To process the RNA samples, we used the Beckman Biomek FX TPE System (Beckman Coulter Inc., Indianapolis) by the manufacturer HT-IVT express 2.0.6 standard protocol from Thermo Fischer Scientific.
 
Hybridization protocol RNA was hybridized and scanned to the Human Genome U219 96-array plate using the GeneTitanR instrument (Thermo Fischer Scientific).
Scan protocol RNA was hybridized and scanned to the Human Genome U219 96-array plate using the GeneTitanR instrument (Thermo Fischer Scientific).
Description healthy control
Data processing All statistical analyses were carried out using the R system for statistical computing, version 3.2.3, whereas support vector machines (SVM) were trained and evaluated using the R package 'e1071', version 1.6-8. We used Ingenuity pathway analysis (IPA) to collect more information about signaling pathways, networks and gene functions
 
Submission date Feb 11, 2020
Last update date Feb 12, 2020
Contact name Pia Moinzadeh
E-mail(s) [email protected]
Organization name University of Cologne
Street address Kerpener Str 62
City Cologne
ZIP/Postal code 50937
Country Germany
 
Platform ID GPL13667
Series (1)
GSE145120 Gene expression data of different SSc subsets

Data table header descriptions
ID_REF
VALUE normalized signal intensity

Data table
ID_REF VALUE
11715100_at 2.724122877
11715101_s_at 2.262941116
11715102_x_at 2.476958502
11715103_x_at 3.390620364
11715104_s_at 2.628337057
11715105_at 1.75455736
11715106_x_at 2.647549822
11715107_s_at 2.256136971
11715108_x_at 2.027817414
11715109_at 1.878706994
11715110_at 5.474294453
11715111_s_at 4.293645398
11715112_at 2.021147786
11715113_x_at 2.905459519
11715114_x_at 3.107266977
11715115_s_at 1.927352673
11715116_s_at 2.273919401
11715117_x_at 1.621172388
11715118_s_at 2.138646129
11715119_s_at 2.327340567

Total number of rows: 49386

Table truncated, full table size 1226 Kbytes.




Supplementary file Size Download File type/resource
GSM4306909_20_02_2013_H12_S29.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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