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Sample GSM4306911 Query DataSets for GSM4306911
Status Public on Feb 12, 2020
Title 20_02_2013_H10_S30.CEL healthy control
Sample type RNA
 
Source name whole blood
Organism Homo sapiens
Characteristics tissue: blood
disease state: healthy control
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using a PAXGene Blood RNA kit (PreAnalytiX GmbH) according to the manufacturer’s instructions.
Label biotin
Label protocol Gene expression analysis was performed using the Thermo Fischer Scientific Human Genome U219 96-array plate. To process the RNA samples, we used the Beckman Biomek FX TPE System (Beckman Coulter Inc., Indianapolis) by the manufacturer HT-IVT express 2.0.6 standard protocol from Thermo Fischer Scientific.
 
Hybridization protocol RNA was hybridized and scanned to the Human Genome U219 96-array plate using the GeneTitanR instrument (Thermo Fischer Scientific).
Scan protocol RNA was hybridized and scanned to the Human Genome U219 96-array plate using the GeneTitanR instrument (Thermo Fischer Scientific).
Description healthy control
Data processing All statistical analyses were carried out using the R system for statistical computing, version 3.2.3, whereas support vector machines (SVM) were trained and evaluated using the R package 'e1071', version 1.6-8. We used Ingenuity pathway analysis (IPA) to collect more information about signaling pathways, networks and gene functions
 
Submission date Feb 11, 2020
Last update date Feb 12, 2020
Contact name Pia Moinzadeh
E-mail(s) [email protected]
Organization name University of Cologne
Street address Kerpener Str 62
City Cologne
ZIP/Postal code 50937
Country Germany
 
Platform ID GPL13667
Series (1)
GSE145120 Gene expression data of different SSc subsets

Data table header descriptions
ID_REF
VALUE normalized signal intensity

Data table
ID_REF VALUE
11715100_at 2.275511099
11715101_s_at 2.649582576
11715102_x_at 2.617611142
11715103_x_at 3.090146529
11715104_s_at 2.100878085
11715105_at 1.878006738
11715106_x_at 1.679435008
11715107_s_at 2.239279698
11715108_x_at 1.865342275
11715109_at 1.956215695
11715110_at 4.873990677
11715111_s_at 4.503762171
11715112_at 2.172892946
11715113_x_at 2.944624381
11715114_x_at 3.291004926
11715115_s_at 1.699263913
11715116_s_at 1.987982764
11715117_x_at 1.629050723
11715118_s_at 2.082964647
11715119_s_at 2.101906347

Total number of rows: 49386

Table truncated, full table size 1225 Kbytes.




Supplementary file Size Download File type/resource
GSM4306911_20_02_2013_H10_S30.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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