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Sample GSM4306912 Query DataSets for GSM4306912
Status Public on Feb 12, 2020
Title 22_02_2013_F09_S31.CEL healthy control
Sample type RNA
 
Source name whole blood
Organism Homo sapiens
Characteristics tissue: blood
disease state: healthy control
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using a PAXGene Blood RNA kit (PreAnalytiX GmbH) according to the manufacturer’s instructions.
Label biotin
Label protocol Gene expression analysis was performed using the Thermo Fischer Scientific Human Genome U219 96-array plate. To process the RNA samples, we used the Beckman Biomek FX TPE System (Beckman Coulter Inc., Indianapolis) by the manufacturer HT-IVT express 2.0.6 standard protocol from Thermo Fischer Scientific.
 
Hybridization protocol RNA was hybridized and scanned to the Human Genome U219 96-array plate using the GeneTitanR instrument (Thermo Fischer Scientific).
Scan protocol RNA was hybridized and scanned to the Human Genome U219 96-array plate using the GeneTitanR instrument (Thermo Fischer Scientific).
Description healthy control
Data processing All statistical analyses were carried out using the R system for statistical computing, version 3.2.3, whereas support vector machines (SVM) were trained and evaluated using the R package 'e1071', version 1.6-8. We used Ingenuity pathway analysis (IPA) to collect more information about signaling pathways, networks and gene functions
 
Submission date Feb 11, 2020
Last update date Feb 12, 2020
Contact name Pia Moinzadeh
E-mail(s) [email protected]
Organization name University of Cologne
Street address Kerpener Str 62
City Cologne
ZIP/Postal code 50937
Country Germany
 
Platform ID GPL13667
Series (1)
GSE145120 Gene expression data of different SSc subsets

Data table header descriptions
ID_REF
VALUE normalized signal intensity

Data table
ID_REF VALUE
11715100_at 2.068054582
11715101_s_at 2.600202905
11715102_x_at 2.409775592
11715103_x_at 3.062675791
11715104_s_at 2.194281029
11715105_at 2.150449091
11715106_x_at 2.01241298
11715107_s_at 2.141746527
11715108_x_at 2.298635348
11715109_at 2.189595272
11715110_at 3.939299087
11715111_s_at 4.054254677
11715112_at 2.511523914
11715113_x_at 3.244175208
11715114_x_at 3.533545866
11715115_s_at 1.620522458
11715116_s_at 2.250576543
11715117_x_at 2.01321319
11715118_s_at 2.08796211
11715119_s_at 2.272883081

Total number of rows: 49386

Table truncated, full table size 1226 Kbytes.




Supplementary file Size Download File type/resource
GSM4306912_22_02_2013_F09_S31.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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