|
| Status |
Public on Mar 11, 2020 |
| Title |
CON-male, pool |
| Sample type |
RNA |
| |
|
| Source name |
3 week old, spleen
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
strain: DBA/1
tissue: spleen
maternal exposure: Control
number of animals: 4
|
| Treatment protocol |
pregnant mice were exposed to BBP via drinking water starting one week before mating until weaning when pups were 3 weeks old, Contols revceived normal drinking water.
|
| Growth protocol |
3 week old offspring from mothers exposed toward BBP in perinatal period
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted using Rneasy Mini Kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
TP_CON_M_pool
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Mar 10, 2020 |
| Last update date |
Mar 11, 2020 |
| Contact name |
Tobias Polte |
| E-mail(s) |
[email protected]
|
| Organization name |
Helmholtz Centre for Environmental Research GmbH - UFZ
|
| Street address |
Permoserstrasse 15
|
| City |
Leipzig |
| ZIP/Postal code |
04318 |
| Country |
Germany |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE146699 |
Gene expression data from splenocytes of 3 week-old offspring after maternal exposure to benzyl butyl phthalate (BBP) |
|
