|
| Status |
Public on Oct 30, 2010 |
| Title |
parnate/mAMFGi, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
parnate/mAMFGi
|
| Organism |
Mus musculus |
| Characteristics |
cell type: converted mouse ESCs
|
| Growth protocol |
ES cells were cultured at 37°C with 5% CO2. mES cells were maintained on irradiated MEF feeders in Dulbecco’s modified Eagle medium (DMEM; GIBCO), supplemented with 10% fetal bovine serum (FBS; GIBCO), 0.1 mM Beta-mercaptoethanol (GIBCO), 2 mM L-glutamine, 0.1 mM MEM nonessential amino acid, and 1,000 units/ml of LIF (Chemicon).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and purified with RNeasy Plus minikit (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
Biotin-16-UTP-labeled cRNA was synthesized from 500 ng total RNA with the Illumina TotalPrep RNA amplification kit (Ambion AMIL1791, Foster City, CA, USA).
|
| |
|
| Hybridization protocol |
The hybridization mix containing 750 ng of labeled amplified cRNA was prepared according to the Illumina BeadStation 500X System Manual (Illumina, San Diego, CA, USA) using the supplied reagents and GE Healthcare Streptavidin-Cy3 staining solution. Hybridization to the Illumina Array MouseRef-8 v2 was for 18 h at 55 °C on a BeadChip Hyb Wheel.
|
| Scan protocol |
The array was scanned using the Illumina BeadArray Reader.
|
| Description |
Gene expression data from converted cells
|
| Data processing |
The data were analyzed using Bead Array suite software. Rank invariant normalization method was chosen to obtain the signal intensity.
|
| |
|
| Submission date |
Aug 14, 2009 |
| Last update date |
Mar 20, 2010 |
| Contact name |
Hongyan Zhou |
| Organization name |
the scripps research institute
|
| Street address |
10550 North Torrey Pines Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE17664 |
Conversion of Epiblast Stem Cells to Embryonic Stem Cells by Small Molecules |
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