|
| Status |
Public on Jun 04, 2020 |
| Title |
RNAseq_St15_controlRNAi_female_embryos_04 |
| Sample type |
SRA |
| |
|
| Source name |
st15 embryos
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: progeny of mat-a-tubGal4/controlRNAi mothers crossed to their siblings (Bloomington stocks: 7062 & 36304) expressed in Sxl-GFP genetic background; Sorted to be GFP positive
tissue: st15 embryos
Sex: female
|
| Growth protocol |
D. melanogaster flies were maintained in standard Drosophila medium at 25oC.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
st15 embryos: 14-16h embryos were inspected under the stereoscope and were selected for the desired stages and for Sxl-GFP presence or absence to distinguish their gender. 25 embryos were homogenized in Trizol and total mRNA was isolated using the microRNA Zymo Kit. RNA Quality was assessed by Fragment Analyzer.
TruSeq Stranded mRNA.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Description |
st15_controlRNAi_females_rep4
processed data file: st15_RNAseq_counts.txt
|
| Data processing |
Quality control, mapping and count retrieval were done as described in Bhardwaj, V. et al. "snakePipes: facilitating flexible, scalable and integrative epigenomic analysis" https://doi.org/10.1093/bioinformatics/btz436
Genome_build: dm6 (Release 6 plus ISO1 MT)
Supplementary_files_format_and_content: *_counts.tsv: Tab-delimited text files include gene counts.
|
| |
|
| Submission date |
Mar 24, 2020 |
| Last update date |
Jun 04, 2020 |
| Contact name |
Asifa Akhtar |
| E-mail(s) |
[email protected]
|
| Organization name |
Max Planck Institute of Immunobiology and Epigenetics
|
| Department |
Chromatin Regulation
|
| Lab |
Akhtar Lab
|
| Street address |
Stuebeweg 51
|
| City |
Freiburg |
| ZIP/Postal code |
79108 |
| Country |
Germany |
| |
|
| Platform ID |
GPL23323 |
| Series (2) |
| GSE130333 |
H4K16ac developmental dynamics during Drosophila development [RNA] |
| GSE130335 |
Intergenerationally maintained Histone H4 lysine 16 acetylation is instructive for future gene activation |
|
| Relations |
| BioSample |
SAMN14440780 |
| SRA |
SRX7982548 |
