C + S: Context + Shock. Young male C57BL/6J mice were exposed to a contextual fear conditioning paradigm that consisted of: Placement into a novel spatial context for 2 min. After 2 min, a 1 sec (0.5 mA) shock was administered through a floor grid. The 2 min-1 sec shock paradigm was repeated for a total of 3 shocks. 1 min after the last shock, animals were removed to their homecage. RNA was extracted was extracted 4 hour after the last shock treatment.
