|
| Status |
Public on May 17, 2021 |
| Title |
GK21 |
| Sample type |
RNA |
| |
|
| Source name |
papillary thyroid carcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
histological variant: Classical
tissue type: Primary tumor
collection before/after rai: Before
patient id: pt_1
patient rai responce: Avid
rai uptake at the metastatic site: Yes
disease: Remission
lesion by ptc-ma: WT
lesion class: WT
patients with available multiple tumor tissues: No
tumor purity class by cibersort: high purity
|
| Treatment protocol |
Patients RAI treatment following tumor surgical resection (thyroidectomy)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from snap-frozen tissues using Qiagen miRNeasy Mini Kit and quantified by Qubit 2.0 Fluorometer (Thermo Fisher).
|
| Label |
Cy3
|
| Label protocol |
RNA was labeled and processed according to manufacturer’s recommended protocols.
|
| |
|
| Hybridization protocol |
Hybridization was performed according to manufacturer’s standard protocols.
|
| Scan protocol |
miRNA microarrays were scanned with Agilent SureScan Microarray Scanner
|
| Description |
1_P
microRNA expression profiles from papillary thyroid carcinoma
|
| Data processing |
Scanned images were processed with Agilent Feature Extraction v10.7 with default parameters to obtain raw data. Raw data were imported in R and processed using the limma package. Spot-level raw intensities were background-corrected using the normexp method and normalized with quantile normalization.
|
| |
|
| Submission date |
May 26, 2020 |
| Last update date |
May 17, 2021 |
| Contact name |
Matteo Dugo |
| E-mail(s) |
[email protected]
|
| Organization name |
IRCCS Ospedale San Raffaele
|
| Department |
Department of Medical Oncology
|
| Street address |
via Olgettina 60
|
| City |
Milan |
| ZIP/Postal code |
20132 |
| Country |
Italy |
| |
|
| Platform ID |
GPL21575 |
| Series (2) |
| GSE151180 |
Gene and miRNA expression in radioiodine refractory and avid papillary thyroid carcinomas (miRNA expresseion dataset) |
| GSE151181 |
Gene and miRNA expression in radioiodine refractory and avid papillary thyroid carcinomas |
|
