|
| Status |
Public on Jul 23, 2024 |
| Title |
HCT116_CER3_6: EI-52 (CER) treated HCT116, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
HCT116
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCT116
treatment: EI-52 (CER)
|
| Treatment protocol |
Cells were plated and treated with DMSO or EI-52 at 8 µM for the indicated times.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
the Nucleospin RNA kit (Macherey-Nagel) was used for total RNA extraction
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA
|
| |
|
| Hybridization protocol |
following fragmentation, 15µg cRNA were hybridized for 16h at 45°C on Human Genome U133 Plus 2.0 array. Genechips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GCS 3000 7G
|
| Description |
Gene expression data from HCT116, treated with EI-52
|
| Data processing |
Gene expression analysis was performed using Array Studio software (Omicsoft Corporation). Raw microarray data (CEL files) were processed using quantile normalization and robust multiarray average algorithm.
|
| |
|
| Submission date |
Jul 03, 2020 |
| Last update date |
Jul 23, 2024 |
| Contact name |
PIERRE SAINTIGNY |
| E-mail(s) |
[email protected]
|
| Organization name |
Centre Léon Bérard
|
| Street address |
28 Rue Laennec
|
| City |
Lyon |
| ZIP/Postal code |
69008 |
| Country |
France |
| |
|
| Platform ID |
GPL22321 |
| Series (1) |
| GSE153759 |
Microarray expression data of HCT116 with treatment (EI-52) or controls (DMSO) |
|
