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Sample GSM476678 Query DataSets for GSM476678
Status Public on Dec 02, 2009
Title Tgd.vg2+.act.Sp#1
Sample type RNA
 
Source name spleen
Organism Mus musculus
Characteristics age: 5 weeks
exclusion markers: CD4- CD8- NK1.1-
genetic background: C57BL/6J
phenotype markers: TCRd+ Vg2+ CD44+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass Tissue Grinder MACS depletion / Flow Sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description Tgd.vg2+.act.Sp#1, SpleenVg2+, activated
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Dec 01, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 17.1933
10344622 264.0678
10344624 250.9392
10344633 700.2813
10344637 178.4353
10344653 19.9863
10344658 197.049
10344674 13.2822
10344679 38.6115
10344707 318.0045
10344713 328.7399
10344723 97.6864
10344725 39.9387
10344741 2234.7111
10344743 30.5851
10344750 112.1438
10344772 30.7042
10344789 112.9518
10344797 444.6231
10344799 208.4661

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM476678_EA07068_54193_MoGene_Tgd.vg2+.act.Sp_#1.cel.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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