|
| Status |
Public on Mar 18, 2010 |
| Title |
Placenta_Spike_Pool2_Agilent |
| Sample type |
RNA |
| |
|
| Source name |
Human Placenta_Spike_Pool2
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Placenta
gender: Female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from placental tissue using the miRVana Isolation Kit (Ambion). Protocol was used in such a way that small RNAs were retained.
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled miRNA was prepared from 100ng Total RNA using the Agilents labeling protocol and Asuragen's internal standard operating procedures.
|
| |
|
| Hybridization protocol |
As per Agilents recommendations.
|
| Scan protocol |
Slides were scanned immediately after washing on the Axon 4000B scanner using one color scan setting (Scan resolution was 5um, Dye channel is set to Green and Green PMT is set to 100%).
|
| Description |
251643610808_002_1_2_GeneView.txt
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.5.3.1 (Agilent) using default parameters (protocol miRNA-v1_95_May07 and Grid: 016436_D_20070426) to obtain background subtracted Processed Signal intensities.
|
| |
|
| Submission date |
Dec 01, 2009 |
| Last update date |
Mar 18, 2010 |
| Contact name |
Sachin Sah |
| Organization name |
Asuragen Inc.
|
| Street address |
2150 Woodward St, Suite 100
|
| City |
Austin |
| State/province |
TX |
| ZIP/Postal code |
78744 |
| Country |
USA |
| |
|
| Platform ID |
GPL9081 |
| Series (1) |
| GSE19248 |
Performance Evaluation of Commercial miRNA Expression Array Platforms |
|
