|
| Status |
Public on Apr 07, 2021 |
| Title |
21-30-batch_1-IL17AF_10_ng_ml |
| Sample type |
RNA |
| |
|
| Source name |
abdominal skin
|
| Organism |
Homo sapiens |
| Characteristics |
sample_id: 21
donor_id: 30
rna_integrity: 8.7
batch: batch_1
treatment: IL17AF_10_ng_ml
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from ex vivo cultured human skin biopsies using the mirVana kit (Life Technologies) according to the manufacturer’s instructions and Precellys® 24 bead-based homogenizer (Precellys® Bertin Technologies, France) for the mechanical lysing of the tissue.
|
| Label |
biotin
|
| Label protocol |
The extracts were labeled with GeneChip™ WT Terminal Labeling Kit according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Hybridizations was performed with the GeneChip™ Hybridization Control Kit according to the manufacturer's instructions.
|
| Scan protocol |
Scanning has been performed according to the GeneTitan manufacturer's instructions.
|
| Data processing |
The normalization and summarization was carried out in R, using RMA (Affy) and robust quantile normalization (preprocessCore)
|
| |
|
| Submission date |
Sep 23, 2020 |
| Last update date |
Apr 09, 2021 |
| Contact name |
Maxim Alexandre Xavier Tollenaere |
| E-mail(s) |
[email protected]
|
| Phone |
+4531327695
|
| Organization name |
LEO Pharma
|
| Department |
Advanced Biologics
|
| Street address |
Industriparken 55
|
| City |
Ballerup |
| ZIP/Postal code |
2750 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL17692 |
| Series (1) |
| GSE158448 |
Multiple IL-17 family cytokines signaling through IL-17RA drive inflammatory pathways in psoriasis |
|
