|
Status |
Public on Apr 07, 2021 |
Title |
44-62-batch_3-IL17C_10_ng_ml |
Sample type |
RNA |
|
|
Source name |
abdominal skin
|
Organism |
Homo sapiens |
Characteristics |
sample_id: 44 donor_id: 62 rna_integrity: 9.1 batch: batch_3 treatment: IL17C_10_ng_ml
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted from ex vivo cultured human skin biopsies using the mirVana kit (Life Technologies) according to the manufacturer’s instructions and Precellys® 24 bead-based homogenizer (Precellys® Bertin Technologies, France) for the mechanical lysing of the tissue.
|
Label |
biotin
|
Label protocol |
The extracts were labeled with GeneChip™ WT Terminal Labeling Kit according to the manufacturer's instructions.
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|
|
Hybridization protocol |
Hybridizations was performed with the GeneChip™ Hybridization Control Kit according to the manufacturer's instructions.
|
Scan protocol |
Scanning has been performed according to the GeneTitan manufacturer's instructions.
|
Data processing |
The normalization and summarization was carried out in R, using RMA (Affy) and robust quantile normalization (preprocessCore)
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|
|
Submission date |
Sep 23, 2020 |
Last update date |
Apr 09, 2021 |
Contact name |
Maxim Alexandre Xavier Tollenaere |
E-mail(s) |
[email protected]
|
Phone |
+4531327695
|
Organization name |
LEO Pharma
|
Department |
Advanced Biologics
|
Street address |
Industriparken 55
|
City |
Ballerup |
ZIP/Postal code |
2750 |
Country |
Denmark |
|
|
Platform ID |
GPL17692 |
Series (1) |
GSE158448 |
Multiple IL-17 family cytokines signaling through IL-17RA drive inflammatory pathways in psoriasis |
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