|
| Status |
Public on May 17, 2021 |
| Title |
Young_PD-1KO with tumor |
| Sample type |
RNA |
| |
|
| Source name |
CD8 T-cells from draining lymph node
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: PD-1-/-
age: 2 months old
tumor cell injection: MC38 cell injection
tissue: Lymph node
|
| Treatment protocol |
No treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were isolated from cells using Nucleospin RNA (Macherey-Nagel) and used for cDNA synthesis using RevatraAce reverse transcriptase (Toyobo) and random primer according to the manufacture’s instructions.
|
| Label |
Cy3
|
| Label protocol |
labeled cRNA were prepared from 1~5 ug total RNA using the Agilent’s Quick Amp Labeling Kit
|
| |
|
| Hybridization protocol |
Following fragmentation, 1.65 ug of cRNA were hybridized to the Agilent expression microarray according to the protocols provided by the manufacturer
|
| Scan protocol |
Arrays were scanned using the Agilent Technologies G4900DA SG12494263
|
| Data processing |
Array data export processing and analysis was performed using Agilent Feature Extraction v11.0.1.1 . Array probes that have Flag A in samples were filtered out. Selected gProcessedSignal value was transformed by logarithm and normalized by quantile method.
|
| |
|
| Submission date |
Nov 17, 2020 |
| Last update date |
May 19, 2021 |
| Contact name |
Kenji Chamoto |
| Organization name |
Graduate School of Medicine, Kyoto University
|
| Department |
Department of Immunology and Genomic Medicine
|
| Street address |
Yoshida Konoe-cho, Sakyo-ku
|
| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
606-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21163 |
| Series (1) |
| GSE161659 |
Aging affects gene expression in CD8 T-cells from mice with or without MC38 tumors |
|
