|
| Status |
Public on Jul 20, 2021 |
| Title |
Young TIG-3 |
| Sample type |
RNA |
| |
|
| Source name |
TIG-3 41 PDL
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: TIG-3
cell type: Normal human diploid fibroblasts
age: Young
|
| Growth protocol |
Cells were cultured in DMEM containing 10% FBS at 37°C 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were purified using ISOGEN II.
|
| Label |
Cy5
|
| Label protocol |
RNA samples were labelled with Cy5 using Amersham Cy5 Mono-Reactive Dye.
|
| |
|
| Hybridization protocol |
RNA samples were hybridized onto 3D-Gene human oligo chip 25k V2.1.
|
| Scan protocol |
Oligo chips were scanned by a 3D-Gene scanner.
|
| Data processing |
The raw signal intensity was subtracted with the background mean and normalized to the value in which the median intensity was 25.
|
| |
|
| Submission date |
Nov 26, 2020 |
| Last update date |
Jul 20, 2021 |
| Contact name |
Kimiyoshi Yano |
| Organization name |
Hiroshima University
|
| Street address |
Kasumi 1-2-3, Minami-ku
|
| City |
Hiroshima |
| ZIP/Postal code |
734-8553 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13915 |
| Series (1) |
| GSE162201 |
Gene expression profiles in replicative senescence in TIG-3 cells |
|
