|
| Status |
Public on Jan 14, 2010 |
| Title |
A2BAR -/- mouse heart, control, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
heart tissue, untreated controls
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
strain: BL6/C57
genotype/variation: A2BAR-/-
age: 12 weeks
|
| Treatment protocol |
In situ IP was performed in A2BAR-/- mice and matched littermate controls. IP consisted of 4 cycles of 5 min myocardial ischemia and 5 min reperfusion followed by 2 h of reperfusion. The area at risk was excised and shock frozen in liquid nitrogen. Controls were sham operated.
|
| Growth protocol |
male wildtype BL6/C57 mice were ordered from Jackson Laboratories. A2BAR deficient mice (BL6/C57 background) were bred in house. 12 week old male were used for the study.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
After hybridization and staining, the arrays were scanned using a GCS3000 with the latest 7G upgrade
|
| Description |
snap frozen, left ventricle, area at risk
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jan 13, 2010 |
| Last update date |
Jan 13, 2010 |
| Contact name |
Tobias Eckle |
| E-mail(s) |
[email protected]
|
| Phone |
72-949-5646
|
| Organization name |
UC Denver
|
| Department |
Anesthesiology
|
| Street address |
12700 E 19th Avenue
|
| City |
Denver |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE19875 |
Expression data from murine hearts exposed to ischemic preconditioning comparing A2BAR null and wildtype BL6/C57 mice |
|
