|
| Status |
Public on May 01, 2021 |
| Title |
BD++ |
| Sample type |
SRA |
| |
|
| Source name |
CD3+Vγ4+ KN6 γδ T-cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue origin: Adult Thymus
genotype: RBPJ Inducible; KN6 Transgenic
|
| Growth protocol |
For isolation of BD and DD samples, KN6 cells were sorted from 8-10 week old adult KN6 Transgenic mice. For isolation of BD++, BD+-, DD++, and DD+- samples, KN6 cells were sorted from 8-10 week old adult RBPJ Inducible; KN6 Transgenic mice that were initially treated with doxycycline for 8 days, followed by 4 days of continued doxycycline treatment (++ samples) or 4 days of discontinued doxycycline treatment (+- samples).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
CD3+Vγ4+ KN6 cells were sorted from thymi using BD FACSAria Fusion.
Library construction was done using 10x Genomics Chromium Controller v3.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
RNA sequencing was performed using Illumina NovaSeq 6000.
Raw data were aligned to GRCm38 using Cell Ranger 5.0.
Raw read counts were obtained using Cell Ranger 5.0.
Genome_build: GRCm38.
Supplementary_files_format_and_content: .tsv and .mtx files.
|
| |
|
| Submission date |
Feb 01, 2021 |
| Last update date |
May 02, 2021 |
| Contact name |
Juan Carlos Zuniga-Pflucker |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Toronto
|
| Department |
Immunology
|
| Street address |
2075 Bayview Avenue
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M4N 3M5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE165908 |
Single-Cell RNA Sequencing of Thymic KN6 γδ T-Cells Modulated by TCR Signal Strength and Notch Signaling |
|
| Relations |
| BioSample |
SAMN17727478 |
| SRA |
SRX9995795 |
