|
| Status |
Public on Mar 08, 2010 |
| Title |
ZBTB20-KO P2 hippocampus rep1 |
| Sample type |
RNA |
| |
|
| Source name |
P2 knockout hippocampus
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Hippocampus
genotype: ZBTB20 knockout
age: P2
|
| Treatment protocol |
After anesthesia on ice, hippocampi were manually dissected under the dissecting scope and snap-frozen in liquid nitrogen before storage at -80C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
|
| Description |
Gene expression data from P2 hippocampus.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Feb 05, 2010 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Weiping J Zhang |
| Organization name |
Second Military Medical University
|
| Department |
Pathophysiology
|
| Street address |
800 Xiangyin Road
|
| City |
Shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE19513 |
Expression data from P2 mouse hippocampus |
|
| Relations |
| Reanalyzed by |
GSE119085 |
