|
Status |
Public on Mar 01, 2011 |
Title |
Dnmt3L wildtype spermatids 3 |
Sample type |
RNA |
|
|
Source name |
Mouse round spermatids
|
Organism |
Mus musculus |
Characteristics |
strain: C57Bl/6 age: 8-12 weeks tissue: Testis genome/variation: wildtype cell type: spermatids
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. RNA quality was assessed with Agilent Bioanalyser.
|
Label |
Cy3
|
Label protocol |
Standard Illumina protocol
|
|
|
Hybridization protocol |
Standard Illumina protocol
|
Scan protocol |
Standard Illumina protocol
|
Description |
no additional information
|
Data processing |
Un-normalized summary probe profiles were output from BeadStudio for two Illumina Mouse Whole-Genome Version 1.1 BeadChips. The raw intensities were transformed by variance stabilising transformation (vst) and quantile normalised using the lumi package of the Bioconductor open-source software project (http://www.bioconductor.org).
|
|
|
Submission date |
Feb 09, 2010 |
Last update date |
Mar 01, 2011 |
Contact name |
Gordon K Smyth |
E-mail(s) |
[email protected]
|
Phone |
(+61 3) 9345 2326
|
Fax |
(+61 3) 9347 0852
|
URL |
http://www.wehi.edu.au
|
Organization name |
Walter and Eliza Hall Institute of Medical Research
|
Department |
Bioinformatics
|
Lab |
Smyth
|
Street address |
1G Royal Pde
|
City |
Parkville |
State/province |
Vic |
ZIP/Postal code |
3052 |
Country |
Australia |
|
|
Platform ID |
GPL6481 |
Series (1) |
GSE20241 |
Genome-wide analysis of Dnmt3L heterozygous and wildtype spermatocytes and spermatids |
|