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Sample GSM507268 Query DataSets for GSM507268
Status Public on Mar 01, 2011
Title Dnmt3L wildtype spermatids 3
Sample type RNA
 
Source name Mouse round spermatids
Organism Mus musculus
Characteristics strain: C57Bl/6
age: 8-12 weeks
tissue: Testis
genome/variation: wildtype
cell type: spermatids
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. RNA quality was assessed with Agilent Bioanalyser.
Label Cy3
Label protocol Standard Illumina protocol
 
Hybridization protocol Standard Illumina protocol
Scan protocol Standard Illumina protocol
Description no additional information
Data processing Un-normalized summary probe profiles were output from BeadStudio for two Illumina Mouse Whole-Genome Version 1.1 BeadChips. The raw intensities were transformed by variance stabilising transformation (vst) and quantile normalised using the lumi package of the Bioconductor open-source software project (http://www.bioconductor.org).
 
Submission date Feb 09, 2010
Last update date Mar 01, 2011
Contact name Gordon K Smyth
E-mail(s) [email protected]
Phone (+61 3) 9345 2326
Fax (+61 3) 9347 0852
URL http://www.wehi.edu.au
Organization name Walter and Eliza Hall Institute of Medical Research
Department Bioinformatics
Lab Smyth
Street address 1G Royal Pde
City Parkville
State/province Vic
ZIP/Postal code 3052
Country Australia
 
Platform ID GPL6481
Series (1)
GSE20241 Genome-wide analysis of Dnmt3L heterozygous and wildtype spermatocytes and spermatids

Data table header descriptions
ID_REF
VALUE lumi (bioc) normalized log2 signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
102450270 6.746850316 0.4816
104200035 6.765805117 0.40923
100730138 6.859376349 0.16906
104230390 6.970733918 0.05614
102630735 6.746850316 0.47848
100450435 6.789589424 0.31815
101230594 6.721804595 0.60262
100780541 6.74504379 0.49033
104200184 6.668470427 0.83718
105360286 6.73738818 0.53712
103060411 6.72464039 0.58765
100630193 6.71904129 0.62508
105270286 6.767559208 0.40362
101690685 6.698700004 0.72739
100060180 6.815988668 0.24766
107100563 6.660184784 0.8665
100450451 6.692270108 0.75546
100610092 6.705941236 0.6937
104810452 13.06005905 0
6840019 9.366155027 0

Total number of rows: 46643

Table truncated, full table size 1256 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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