treatment: c dose (um): 0 time (hr): 48 batch: 1 liver: 1002 reps: 1 cell type: primary hepatocytes
Biomaterial provider
University of Pittsburgh
Growth protocol
Culture protocol: hepatocytes were cultured as described in Gross-Steinmeyer K, Stapleton PL, Tracy JH, Bammler TK, Strom SC, Buhler DR, Eaton DL. Modulation of aflatoxin B1-mediated genotoxicity in primary cultures of human hepatocytes by diindolylmethane, curcumin and xanthohumols.Toxicol Sci. 2009, 112:303-10. Isolation protocol: primary hepatocytes were isolated as described in Strom, S. C., Pisarov, L. A., Dorko, K., Thompson, M. T., Schuetz, J. D., and Schuetz, E. G. (1996). Use of human hepatocytes to study P450 gene induction. Meth. Enzymol. 272, 388-401.
Extracted molecule
total RNA
Extraction protocol
RNA was extracted with the RNeasy micro kit (Qiagen, Valencia, CA).
Label
biotin
Label protocol
For microarray hybridization, RNA was processed for hybridization to GE Healthcare/Amersham Biosciences Arrays according to the manufacturer's instructions.
Hybridization protocol
The standard hybridization protocol was used as recommended by GE Healthcare/Amersham Biosciences CodeLink UniSet Human 20K I Bioarray.
Scan protocol
GeneChips were scanned using the Axon 4000B scanner.
Description
primary hepatocytes
Data processing
CodeLink System Software - Analysis, version: 2.3.2. R/Bioconductor quantile normalized, no background subtraction, Imagene Signal.Mean, log2
