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Sample GSM51412 Query DataSets for GSM51412
Status Public on May 19, 2005
Title CB2_RLO_U133B
Sample type RNA
 
Source name umbilical cord blood CD34+CD38-CD33-Rho(lo)c-kit+ cells
Organism Homo sapiens
Extracted molecule total RNA
 
Description Human UCB from full-term delivered infants was obtained after informed consent in accordance with guidelines approved by the University of Minnesota Committee on the Use of Human Subjects in Research. Each biologically-distinct replicate was comprised of one to four donors that were pooled prior to processing and microarray analysis. CD34+CD38-CD33-Rho(lo)c-kit+ and CD34+CD38-CD33-Rho(hi) fractions were selected by sequential Ficol-Hypaque separation, MACS column depletion and fluorescence activated cell sorting. Total cellular RNA was isolated using the PicoPure RNA Isolation Kit (Arcturus, Mountain View, California) per the manufacturer’s instructions. Seven to ten thousand Rho(lo) and Rho(hi) cells were sorted directly into 100 mcL Extraction Buffer (XB) provided with the PicoPure RNA Isolation Kit (Arctutus) prior to RNA isolation. Labeled complimentary-RNA (cRNA) was generated by one round of IVT-based, linear amplification using the RiboAmp OA RNA Amplification Kit (Arcturus) followed by labeling with the Enzo Bioarray™ HighYield™ RNA Transcript Labeling Kit (Enzo Life Sciences, Farmingdale, New York) according to manufacturer’s instructions. Affymetrix® HG-U133 GeneChips™ were processed using Genedata Refiner software (GeneData, San Francisco, California) to assess overall quality. Feature intensities for each chip were condensed into a single intensity value per gene using the Affymetrix Statistical Algorithm (MAS 5.0) with Tau = 0.015, Alpha1 = 0.04, Alpha2 0.06 and a scaling factor of 500.
 
Submission date May 16, 2005
Last update date Oct 28, 2005
Contact name Craig E Eckfeldt
E-mail(s) [email protected]
Phone 612.626.4249
Organization name University of Minnesota
Department Stem Cell Institute
Street address 420 Delaware St. SE, MMC 806
City Minneapolis
State/province MN
ZIP/Postal code 55455
Country USA
 
Platform ID GPL97
Series (1)
GSE2666 Human Hematopoietic Stem Cell (HSC) and Progenitor Cell (HPC) Expression Profilies

Data table header descriptions
ID_REF
VALUE signal intensity
DETECTION P-VALUE

Data table
ID_REF VALUE DETECTION P-VALUE
200000_s_at 1210.01 0.01855
200001_at 948.43 0.0376
200002_at 2351.75 2.44E-04
200003_s_at 2726.4 2.44E-04
200004_at 5829.35 2.44E-04
200005_at 10286.16 2.44E-04
200006_at 8031.22 2.44E-04
200007_at 8513.61 2.44E-04
200008_s_at 831.17 0.00415
200009_at 18537.02 2.44E-04
200010_at 5207.12 0.001953
200011_s_at 1051.25 0.001221
200012_x_at 41478.23 2.44E-04
200013_at 9222.35 7.32E-04
200014_s_at 7264.32 2.44E-04
200015_s_at 7030.53 2.44E-04
200016_x_at 137103.22 2.44E-04
200017_at 19222.58 0.001953
200018_at 47243.04 2.44E-04
200019_s_at 41178.93 2.44E-04

Total number of rows: 22645

Table truncated, full table size 540 Kbytes.




Supplementary file Size Download File type/resource
GSM51412.CEL.gz 3.2 Mb (ftp)(http) CEL
GSM51412.EXP.gz 471 b (ftp)(http) EXP

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