|
| Status |
Public on Feb 14, 2011 |
| Title |
[CD4+ T-Cells]_[RA]_[2] |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Spleen, peripheral lymph nodes, mesenteric lymph nodes
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Spleen + Lymph Nodes
cell type: CD4+ T cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
Qiagen Total RNA isolation kit
|
| Label |
Biotin (as a biotinylated nucleotide)
|
| Label protocol |
Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000 7G enabled forHigh-Resolution Scanning. The instruments were controlled using GCOS version1.4 software.
|
| Description |
Mouse CD4 T cells were cultured in vitro to obtain RNA for the assay
|
| Data processing |
The data were condensed with MAS 5.0 using the GeneChip Operation Software.
|
| |
|
| Submission date |
Feb 23, 2010 |
| Last update date |
Feb 14, 2011 |
| Contact name |
Chang Kim |
| E-mail(s) |
[email protected]
|
| Phone |
765-494-0976
|
| Organization name |
Purdue
|
| Department |
Comparative Pathobiology
|
| Lab |
Immunology and Hematopoiesis
|
| Street address |
725 Harrison St
|
| City |
West Lafayette |
| State/province |
IN |
| ZIP/Postal code |
47907 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE20500 |
T cell genes regulated by retinoic acid |
|
