|
| Status |
Public on May 23, 2022 |
| Title |
IHCC1-2 |
| Sample type |
SRA |
| |
|
| Source name |
HCC
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: liver
cell type: HCC
|
| Growth protocol |
Primary human hepatocytes from five different donors were purchased from Bioreclamation IVT.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA from primary PHH or iHCC was prepared according to the TruSeqTM RNA Sample Preparation Guide.
RNA libraries were prepared for sequencing using standard Illumina protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Description |
rawCountsHCC.csv
|
| Data processing |
Sequenced reads were trimmed for adaptor sequence and masked for a low complexity or low-quality sequence. The number of raw reads mapped to genes was calculated by RSEM (rsem-1.2.4), and the sample results were put together and normalized by EDAseq (1.99.1).
Genome_build: not provided
Supplementary_files_format_and_content: rawCountsHCC.csv: Comma-separated text file
|
| |
|
| Submission date |
Mar 13, 2021 |
| Last update date |
May 23, 2022 |
| Contact name |
zhiwu jiang |
| E-mail(s) |
[email protected]
|
| Organization name |
GIBH
|
| Street address |
190 Kai Yuan Avenue, Science Park
|
| City |
Guangzhou |
| ZIP/Postal code |
510530 |
| Country |
China |
| |
|
| Platform ID |
GPL23227 |
| Series (1) |
| GSE168852 |
RNA-seq analysis of HCC and hepatocytes |
|
| Relations |
| BioSample |
SAMN18296504 |
| SRA |
SRX10337416 |
