|
| Status |
Public on Jun 26, 2010 |
| Title |
GST RNAi, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
SL2 cells, GST RNAi
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell line: SL2
rnai target: GST
|
| Treatment protocol |
RNA interference (RNAi) of target genes were carried out as described previously (Straub et al., 2005). In brief, 1.5 10^6 SL2 cells were incubated with 10 μg dsRNA for 1 hour in serum-free medium. After addition of serum-containing medium, cells were incubated for 7 days at 26°C before RNA extraction.
|
| Growth protocol |
SL2 cells were cultivated as previously described (Straub et al. 2005).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Trizol (Invitrogen) followed by a purification using RNeasy kit (Quiagen) according to the instructions of the suppliers.
|
| Label |
biotin
|
| Label protocol |
Performed by imaGenes (www.imagenes-bio.de).
|
| |
|
| Hybridization protocol |
Performed by imaGenes (www.imagenes-bio.de).
|
| Scan protocol |
Performed by imaGenes (www.imagenes-bio.de).
|
| Description |
gst3
|
| Data processing |
Intensity values were normalized, summarized and log2 transformed using the ‘gcrma’ package (Wu and Irizarry, 2005).
|
| |
|
| Submission date |
Mar 10, 2010 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Christian Feller |
| E-mail(s) |
[email protected]
|
| Phone |
089-2180-75-436
|
| URL |
http://www.molekularbiologie.abi.med.uni-muenchen.de/index.html
|
| Organization name |
Adolf-Butenandt Institute at the LMU University Munich
|
| Department |
Molecular Biology
|
| Lab |
Peter B. Becker
|
| Street address |
Schillerstraße 44
|
| City |
Munich |
| ZIP/Postal code |
80336 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE20744 |
The activation potential of MOF is constrained for dosage compensation, MBD-R2 transcriptome analysis |
|
| Relations |
| Reanalyzed by |
GSE119084 |
