|
| Status |
Public on Apr 19, 2022 |
| Title |
TX1903LHZY2X |
| Sample type |
SRA |
| |
|
| Source name |
colonic epithelial cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: primary colonic epithelial cells
genotype: Mettl14_ KO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Colonic epithelial cells were stained with EpCAM and live/dead cell kit on ice in dark for 30 min. The EpCAM+ live cells were sorted by flow cytometry
The single cell suspension of colonic epithelial cells were processed by 10x Genomics 3'RNASeq V2
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Illumina Bcl2fastq software used for basecalling.
CellRanger was used to analyze the original single cell RNA sequencing results.
Genome_build: mm10
Supplementary_files_format_and_content: text file, raw counts of sequencing reads for the genes of interest after filtering
|
| |
|
| Submission date |
Apr 03, 2021 |
| Last update date |
Apr 19, 2022 |
| Contact name |
Bing Hua Li |
| E-mail(s) |
[email protected]
|
| Organization name |
Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine
|
| Street address |
280 Chongqing South Rd, Building #5, Room 602, Shanghai
|
| City |
Shanghai |
| ZIP/Postal code |
200021 |
| Country |
China |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE171422 |
m6A mRNA modification is a rheostat to maintain colonic epithelial cell homeostasis [scRNA-seq] |
| GSE171423 |
m6A mRNA modification is a rheostat to maintain colonic epithelial cell homeostasis |
|
| Relations |
| BioSample |
SAMN18612580 |
| SRA |
SRX10509236 |
