|
| Status |
Public on Mar 20, 2010 |
| Title |
LEC_AdCTR_24h |
| Sample type |
RNA |
| |
|
| Source name |
LECs, infected with GFP-expressing adenovirus for 24 hours
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Primary lymphatic endothelial cells
|
| Growth protocol |
Standard Endothelial Basal Media (Lonza, Inc), 5% CO2 incubator at 37C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U1333A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from normal human primary lymphatic endothelial cells that were infected with a GFP-expressing adenovirus for 24 hours
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Mar 19, 2010 |
| Last update date |
Mar 19, 2010 |
| Contact name |
Young Kwon Hong |
| E-mail(s) |
[email protected]
|
| Organization name |
Norris Cancer Center
|
| Street address |
1441 Eastlake Ave NOR6336
|
| City |
los angeles |
| State/province |
CA |
| ZIP/Postal code |
90089 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE20978 |
Activated Notch-induced transcriptional profile modulation in human primary dermal lymphatic endothelial cells |
|
