|
| Status |
Public on Aug 31, 2021 |
| Title |
D1: hda4_1st_μG 2 |
| Sample type |
RNA |
| |
|
| Source name |
C.elegans, RB756 hda-4 (ok518), flight µG, 1st generation, replicate 2
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
tag: Adult
genotype: RB756 hda-4 (ok518)
|
| Treatment protocol |
The frozen worms were thawed and washed by M9 buffer.
|
| Growth protocol |
The worms of each generations were cultured under microgravity at the international space station (ISS). The worms were cultured in M9 cholesterol buffer contained E. coli OP50 for 4 days from L1 larva. The cultured worms were frozen at -95 C degrees in ISS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using ISGEN (Nippon gene)following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 200ng RNA using the One-Color Low RNA Input Linear Amplification kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA).
|
| |
|
| Hybridization protocol |
The labeled cRNA (1.65 µg/sample) was hybridized to a microarray using the Agilent Gene Expression Hybridization Kit at 65°C for 17 hours.
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G49000DA) using one color scan setting for 4x44k array slides.
|
| Description |
Gene expression of hda-4 mutant 1st generation in µG condition
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 11.5.1.1
Microarray data were analyzed using Gene Spring software (Agilent Technologies). Normalized data were produced by Normalize to 75 percentile sift protocol. Arrays were filtered on expression (20-100)th percentile in raw data.
|
| |
|
| Submission date |
May 06, 2021 |
| Last update date |
Aug 31, 2021 |
| Contact name |
Akira Higashibata |
| E-mail(s) |
[email protected]
|
| Phone |
050-3362-3898
|
| Organization name |
Japan Aerospace Exploration Agency
|
| Department |
Department of Space Biology and Microgravity Sciences
|
| Lab |
ISS Science Project office
|
| Street address |
2-1-1,Sengen
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8505 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11346 |
| Series (1) |
| GSE173985 |
The effect of microgtavity on the epigenetic modification in C. elegans - JAXA "EPIGENETICS" spaceflight experiment |
|
