|
Status |
Public on Apr 24, 2010 |
Title |
type 2 diabetes 2_microRNA |
Sample type |
RNA |
|
|
Source name |
blood
|
Organism |
Homo sapiens |
Characteristics |
patient type: type 2 diabetes 2 gender: male
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
Hy3
|
Label protocol |
LNA-modified oligonucleotide (Exiqon) probes for human, mouse and rat miRNAs annotated in miRBase version 11.0 were used in the microarray. Total RNA (1 μg) was 3’-end –labeled with Cy3 dye using the miRCURY LNA ™ Power Labeling Kit (Exiqon)
|
|
|
Hybridization protocol |
hybridized on miRCURY LNA™ Arrays, using MAUI® hybridization system
|
Scan protocol |
Chips were scanned on Innopsys Scan 700, microarray scanner at 532 nm
|
Description |
replicate 2
|
Data processing |
miRNA microarray data were analyzed by subtracting the mean background signal (B532nm) from the test signal (F532nm)
|
|
|
Submission date |
Apr 13, 2010 |
Last update date |
Apr 23, 2010 |
Contact name |
Kandiah Jeyaseelan |
E-mail(s) |
[email protected]
|
Organization name |
National University of Singapore
|
Department |
Biochemistry
|
Street address |
8 Medical Drive
|
City |
Singapore |
ZIP/Postal code |
117597 |
Country |
Singapore |
|
|
Platform ID |
GPL10322 |
Series (2) |
GSE21321 |
Blood microRNA profiles and upregulation of hsa-miR-144 in males with type 2 diabetes mellitus. |
GSE26168 |
Type 2 Diabetes mellitus: mRNA and miRNA profiling |
|