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Sample GSM532868 Query DataSets for GSM532868
Status Public on Apr 24, 2010
Title type 2 diabetes 9_microRNA
Sample type RNA
 
Source name blood
Organism Homo sapiens
Characteristics patient type: type 2 diabetes 9
gender: male
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label Hy3
Label protocol LNA-modified oligonucleotide (Exiqon) probes for human, mouse and rat miRNAs annotated in miRBase version 11.0 were used in the microarray. Total RNA (1 μg) was 3’-end –labeled with Cy3 dye using the miRCURY LNA ™ Power Labeling Kit (Exiqon)
 
Hybridization protocol hybridized on miRCURY LNA™ Arrays, using MAUI® hybridization system
Scan protocol Chips were scanned on Innopsys Scan 700, microarray scanner at 532 nm
Description replicate 9
Data processing miRNA microarray data were analyzed by subtracting the mean background signal (B532nm) from the test signal (F532nm)
 
Submission date Apr 13, 2010
Last update date Apr 23, 2010
Contact name Kandiah Jeyaseelan
E-mail(s) [email protected]
Organization name National University of Singapore
Department Biochemistry
Street address 8 Medical Drive
City Singapore
ZIP/Postal code 117597
Country Singapore
 
Platform ID GPL10322
Series (2)
GSE21321 Blood microRNA profiles and upregulation of hsa-miR-144 in males with type 2 diabetes mellitus.
GSE26168 Type 2 Diabetes mellitus: mRNA and miRNA profiling

Data table header descriptions
ID_REF
VALUE F532 Mean - B532 Mean

Data table
ID_REF VALUE
1 -0.003699681
2 0.00180923
3 -0.002552041
4 0.00077528
5 7.206564491
6 6.744037257
7 6.743812955
8 6.300177016
9 -0.009913698
10 -0.001870727
11 -0.004060442
12 -0.001406819
13 -0.003883635
14 0.001781382
15 -0.001847632
16 -4.65545E-05
17 -0.005695397
18 -0.00257778
19 -0.005739206
20 -0.004886442

Total number of rows: 6879

Table truncated, full table size 115 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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