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Sample GSM538235 Query DataSets for GSM538235
Status Public on Apr 29, 2010
Title DC.103+11b-.Lv#2
Sample type RNA
 
Source name liver
Organism Mus musculus
Characteristics age: 8 weeks
genetic background: C57BL/6J
phenotype markers: CD45+ CD11c+ MHC-II+ CD11b low CD103+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Disruption using syringe, ACK lysis
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description DC.103+11b-.Lv#2, liver CD103+ dendritic cells
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 27, 2010
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 20.013
10344622 243.7661
10344624 275.4333
10344633 855.4182
10344637 212.2565
10344653 16.9674
10344658 152.6204
10344674 9.6485
10344679 47.6412
10344707 197.679
10344713 513.8234
10344723 80.4616
10344725 54.4101
10344741 2285.3075
10344743 24.076
10344750 911.2609
10344772 29.9259
10344789 179.8128
10344797 313.406
10344799 323.6694

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM538235_EA07068_96464_MoGene_DC.103+11B-.LV#2.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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