|
| Status |
Public on Apr 29, 2010 |
| Title |
DC.IIhilang-103-11blo.SLN#1 |
| Sample type |
RNA |
| |
|
| Source name |
iliac, axilary and inguinal lymph nodes
|
| Organism |
Mus musculus |
| Characteristics |
age: 10 weeks
exclusion markers: CD4 CD8
genetic background: C57BL/6J
phenotype markers: MHCIIhi CD11c+ Langerin- CD103- CD11b-
separation method: dissection/trimming
Sex: male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
15 minutes digestion with Liberase Blenzyme 3
|
| Label |
Biotin
|
| Label protocol |
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
|
| |
|
| Hybridization protocol |
Affy 1.0 ST Hyb Kit
|
| Scan protocol |
Affymetrix GeneChip Scanner
|
| Description |
DC.IIhilang-103-11blo.SLN#1, skin draining LN MHC IIhi langerin- CD103- CD11b- dendritic cell
|
| Data processing |
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
|
| |
|
| Submission date |
Apr 27, 2010 |
| Last update date |
May 24, 2016 |
| Contact name |
Liang Yang |
| Organization name |
Harvard Medical School
|
| Department |
Pathology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
|
