|
| Status |
Public on Apr 29, 2010 |
| Title |
T.4Mem44h62l.Sp#1 |
| Sample type |
RNA |
| |
|
| Source name |
spleen
|
| Organism |
Mus musculus |
| Characteristics |
age: 6 weeks
exclusion markers: PI CD8 CD11b CD11c CD19 CD49b Gr-1 Ter119 Foxp3
genetic background: C57BL/6J
phenotype markers: CD4 TCRb CD44hi CD62Llo
separation method: dissection/trimming
Sex: male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Glass slide teasing, ACK lysis Two round sort on ARIA
|
| Label |
Biotin
|
| Label protocol |
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
|
| |
|
| Hybridization protocol |
Affy 1.0 ST Hyb Kit
|
| Scan protocol |
Affymetrix GeneChip Scanner
|
| Description |
T.4Mem44h62l.Sp#1, Spleen Memory-Phenotype CD4 cells from GFP-Foxp3-CD45.1-B6 mice
|
| Data processing |
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
|
| |
|
| Submission date |
Apr 27, 2010 |
| Last update date |
May 24, 2016 |
| Contact name |
Liang Yang |
| Organization name |
Harvard Medical School
|
| Department |
Pathology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
|
