|
| Status |
Public on Dec 31, 2011 |
| Title |
Tolerant-drought strA |
| Sample type |
RNA |
| |
|
| Source name |
Tolerant-drought strA
|
| Organism |
Oryza sativa |
| Characteristics |
tissue: leaf
strain: drought-tolerant
stress: drought
|
| Treatment protocol |
Two weeks young seedlings were applied to stress. For salt 50mM NaCl and for drought seedlings were kept outside of media for 24hrs.
|
| Growth protocol |
Samples were grown in hydroponic conditions till two weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol.
|
| Label |
biotin
|
| Label protocol |
According to standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip riceGenome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
According to standard Affymetrix protocol.
|
| Description |
n/a
|
| Data processing |
GCOS version 1.0 signal intensity.
|
| |
|
| Submission date |
May 04, 2010 |
| Last update date |
Dec 31, 2011 |
| Contact name |
Vandna Rai |
| E-mail(s) |
[email protected]
|
| Phone |
+91-01125841787
|
| Organization name |
NRCPB
|
| Department |
Genomics
|
| Lab |
Genomics and proteomics
|
| Street address |
Pusa campus
|
| City |
New Delhi |
| State/province |
Delhi |
| ZIP/Postal code |
110012 |
| Country |
India |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE21651 |
Differential expression for salt and drought stress |
|
