|
| Status |
Public on Mar 02, 2022 |
| Title |
WT-rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Hela_wild-type HBx
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa
genotype/variation: transfected with wild-type HBx
|
| Treatment protocol |
HeLa cell lines expressing wild-type and mutant HBx stably was constructed.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Labeling was performed according to manufacturer's standard protocol.
|
| |
|
| Hybridization protocol |
Sample hybridization was performed for 16 hours at 45°C according to standard protocol. Washing and staining was performed on Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using a GeneChip scanner 3000.
|
| Description |
Gene expression in Hela cells transfected with wild-type HBx
|
| Data processing |
The data were processed with Expression Console Software. Normalization was performed by the Signal Space Transformation-Robust multi-array average method (sst-RMA).
|
| |
|
| Submission date |
Jun 29, 2021 |
| Last update date |
Mar 02, 2022 |
| Contact name |
Xi Chen |
| E-mail(s) |
[email protected]
|
| Organization name |
Second Military Medical University
|
| Street address |
8th Panshan Road
|
| City |
Shanghai |
| ZIP/Postal code |
200082 |
| Country |
China |
| |
|
| Platform ID |
GPL17586 |
| Series (2) |
| GSE179126 |
Expression data from Hela cells stably expressing the wild-type HBx and the four mutant HBx |
| GSE179127 |
Functional study on the mutations of HBV X gene |
|
