|
| Status |
Public on Aug 02, 2011 |
| Title |
human DLBCL tumoral sample, miRNA expr, case 34 |
| Sample type |
RNA |
| |
|
| Source name |
human DLBCL tumoral sample
|
| Organism |
Homo sapiens |
| Characteristics |
case id: 34
tissue: tumoral sample
cell type: diffuse large B cell lymphoma (DLBCL)
status os (0 alive; 1 death): 1
status pfs (0: no event; 1:event:relapse/progression/death): 1
time os (months): 5
time pfs (months): 0
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated by Trizol reagent (Invitrogen, Carlsbad, CA, USA). RNA quality was checked using total RNA (small fraction chip) with the Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Santa Clara, CA) and by 1% agarose electrophoresis, following the standard procedure.
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 0.5 ug RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
|
| |
|
| Hybridization protocol |
100 ng of total RNA were hybridized on an Agilent 8x15K Human miRNA one-color microarray for detecting 470 human and 64 viral miRNAs, following the manufacturer’s instructions (Agilent Technologies)
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 1x44k array slides (Scan Area 61x21.6 mm, Scan resolution 10um, Dye channel is set to Green and Green PMT is set to 100%).
|
| Description |
US22502553_251643611602_S01_miRNA-v1_95_May07_2_3.txt
|
| Data processing |
Between-array median normalization was carried out to render miRNA expression datasets comparable using Genespring GX9 software.
|
| |
|
| Submission date |
May 14, 2010 |
| Last update date |
Aug 02, 2011 |
| Contact name |
Santiago Montes-Moreno |
| E-mail(s) |
[email protected]
|
| Organization name |
CNIO
|
| Department |
Molecular Pathology
|
| Lab |
Lymphoma Group
|
| Street address |
C/ Melchor Fernández Almagro, 3
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28029 |
| Country |
Spain |
| |
|
| Platform ID |
GPL9081 |
| Series (2) |
| GSE21848 |
miRNA profiling of 36 cases of diffuse large B cell lymphoma |
| GSE21849 |
Gene and microRNA expression data of diffuse large B cell lymphoma from human diagnostic frozen samples |
|
