|
| Status |
Public on Mar 14, 2012 |
| Title |
rapid-male-5 |
| Sample type |
RNA |
| |
|
| Source name |
whole blood, globin mRNA reduced, rapid growth, male
|
| Organism |
Homo sapiens |
| Characteristics |
growth: rapid
sex: M
|
| Treatment protocol |
Slow and rapid growth was determined by difference in Z score for weight between term and term plus 12 weeks
|
| Growth protocol |
Whole blood was drawn into PAXgene tubes prior to RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA is purified with the PAXgene Blood RNA kit
|
| Label |
biotin
|
| Label protocol |
After treatment with the GeneChip Globin-Reduction Kit, 2ug of RNA was biotin labeled with the Affymetrix One-Cycle Target Labeling Kit according to manufacturers instructions
|
| |
|
| Hybridization protocol |
The cRNA samples were then fragmented and hybridised onto NuGO-Hs1a520180 (Scienion) Arrays from Affymetrix as performed by ServiceXS (www.servicexs.com)
|
| Scan protocol |
GCOS software (Affymetrix) was used to monitor scanning and to convert the raw image files into cell intensity files (‘.CEL’).
|
| Data processing |
CEL files were processed with the BioConductor gcrma function from the simpleaffy pacakge
|
| |
|
| Submission date |
May 26, 2010 |
| Last update date |
Mar 14, 2012 |
| Contact name |
Simon Cockell |
| E-mail(s) |
[email protected]
|
| Organization name |
Newcastle University
|
| Street address |
Framlington Place
|
| City |
Newcastle |
| ZIP/Postal code |
NE2 4HH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL7020 |
| Series (1) |
| GSE22013 |
Early life growth and gene profile changes during childhood |
|
