|
| Status |
Public on Jun 05, 2010 |
| Title |
hTERT EARLY PASSAGE REP1 |
| Sample type |
RNA |
| |
|
| Source name |
hTERT+ foreskin fibroblasts passage 3
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: foreskin
cell type: early passage immortalized fibroblasts
passage number: 3
|
| Treatment protocol |
BJ-hTERT cells were immortalized by stable transfection with pBabe-hTERT. Replicative senescence was confirmed by morphology change, beta-gal stainng, and growth curve plateau.
|
| Growth protocol |
Fibroblasts were cultured at 37 degrees and 5% CO2 in MEMĪ± supplemented with 1mM sodium pyruvate, 1.5 g/L sodium bicarbonate, and 10% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with mirVana Total RNA Isolation Kit (Ambion).
|
| Label |
Cy3
|
| Label protocol |
Samples were labeled and hybridized as described in Agilent Protocol 1.5.
|
| |
|
| Hybridization protocol |
Samples were labeled and hybridized as described in Agilent Protocol 1.5.
|
| Scan protocol |
Slides were scanned on an Agilent Scanner.
|
| Description |
miRNA expression in early passage immortalized fibroblasts
|
| Data processing |
Mean signal for each probe was quantile normalized and the log2 was taken. Probes were collapsed to individual miRNAs by averaging. Each cell line high passage miRNA array was normalized to its corresponding low passage miRNA array.
|
| |
|
| Submission date |
Jun 04, 2010 |
| Last update date |
Jun 04, 2010 |
| Contact name |
Laura Bonifacio |
| Organization name |
UNC Eshelman School of Pharmacy
|
| Department |
Medicinal Chemistry and Natural Products
|
| Lab |
Jarstfer Lab
|
| Street address |
308 Beard Hall
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27713 |
| Country |
USA |
| |
|
| Platform ID |
GPL9081 |
| Series (1) |
| GSE22134 |
miRNA Expression in Senescent and Immortalized BJ fibroblasts |
|
