|
| Status |
Public on Jun 12, 2010 |
| Title |
2 week iCMs-rep3 |
| Sample type |
RNA |
| |
|
| Source name |
mouse iCMs
|
| Organism |
Mus musculus |
| Characteristics |
strain: CD1
cell type: 2 week iCMs
replicate: 3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PicoPure RNA Isolation Kit (Arcturus)
|
| Label |
Biotin
|
| Label protocol |
Ambion MessageAmp II-biotin enhanced kit (manufacturer's recommendations)
|
| |
|
| Hybridization protocol |
Performed according to the manufacturer's (Affymetrix) recommendations
|
| Scan protocol |
Performed according to the manufacturer's (Affymetrix) recommendations
|
| Description |
mouse reprogramming succeeded fibroblast
|
| Data processing |
Raw intensities from the CEL files were analyzed using Affymetrix Power Tool (APT, version 1.8.5) to generate robust multi-array average intensity in log2 scale for each probe set. The perfect match intensities for each probe set were corrected for background and quantile-normalized using a robust fit of linear models.
|
| |
|
| Submission date |
Jun 10, 2010 |
| Last update date |
Jun 11, 2010 |
| Contact name |
Masaki Ieda |
| E-mail(s) |
[email protected]
|
| Phone |
415-734-2880
|
| Organization name |
Gladstone Institute
|
| Street address |
1650 Owens street,
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94404 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE22292 |
Gene expression in mouse neonatal cardiomyocytes, cardiac fibroblasts, reprogramming failed GFP- cells, and GFP+ iCMs |
|
